l bioreactors (Eppendorf AG)
Eppendorf AG is a verified supplier 
96
Structured Review
Eppendorf AG
l bioreactors
L Bioreactors, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 96/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioflo+320+bioreactor/BioFlo+320/pm42045349-245-4-18
Average 96 stars, based on 108 article reviews
L Bioreactors, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 96/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bioflo+320+bioreactor/BioFlo+320/pm42045349-245-4-18
Average 96 stars, based on 108 article reviews
l bioreactors - by Bioz Stars,
2026-09
96/100 stars
Images
Related Articles
Electrophoresis:Article Title: rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxis Article Snippet: The quantitative measurement of DNA was carried out on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific, USA) at a wavelength of 260 nm. .. Restriction hydrolysis and further electrophoresis in 1% agarose gel were performed to verify the authenticity of the plasmid DNA for comparison with the theoretical length of the restricted fragments. rAAV–B11-Fc was produced by transient transfection of HEK 293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Agarose Gel Electrophoresis:Article Title: rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxis Article Snippet: The quantitative measurement of DNA was carried out on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific, USA) at a wavelength of 260 nm. .. Restriction hydrolysis and further electrophoresis in 1% agarose gel were performed to verify the authenticity of the plasmid DNA for comparison with the theoretical length of the restricted fragments. rAAV–B11-Fc was produced by transient transfection of HEK 293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Plasmid Preparation:Article Title: rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxis Article Snippet: The quantitative measurement of DNA was carried out on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific, USA) at a wavelength of 260 nm. .. Restriction hydrolysis and further electrophoresis in 1% agarose gel were performed to verify the authenticity of the plasmid DNA for comparison with the theoretical length of the restricted fragments. rAAV–B11-Fc was produced by transient transfection of HEK 293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Comparison:Article Title: rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxis Article Snippet: The quantitative measurement of DNA was carried out on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific, USA) at a wavelength of 260 nm. .. Restriction hydrolysis and further electrophoresis in 1% agarose gel were performed to verify the authenticity of the plasmid DNA for comparison with the theoretical length of the restricted fragments. rAAV–B11-Fc was produced by transient transfection of HEK 293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Produced:Article Title: rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxis Article Snippet: The quantitative measurement of DNA was carried out on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific, USA) at a wavelength of 260 nm. .. Restriction hydrolysis and further electrophoresis in 1% agarose gel were performed to verify the authenticity of the plasmid DNA for comparison with the theoretical length of the restricted fragments. rAAV–B11-Fc was produced by transient transfection of HEK 293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Transfection:Article Title: rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxis Article Snippet: The quantitative measurement of DNA was carried out on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific, USA) at a wavelength of 260 nm. .. Restriction hydrolysis and further electrophoresis in 1% agarose gel were performed to verify the authenticity of the plasmid DNA for comparison with the theoretical length of the restricted fragments. rAAV–B11-Fc was produced by transient transfection of HEK 293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Cell Culture:Article Title: rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxis Article Snippet: The quantitative measurement of DNA was carried out on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific, USA) at a wavelength of 260 nm. .. Restriction hydrolysis and further electrophoresis in 1% agarose gel were performed to verify the authenticity of the plasmid DNA for comparison with the theoretical length of the restricted fragments. rAAV–B11-Fc was produced by transient transfection of HEK 293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Stable Transfection:Article Title: Scalable bioprocess for the high-yield production of SARS-CoV-2 trimeric spike protein-based immunogen (IMT-CVAX) using suspension CHO cells Article Snippet: The COVID-19 pandemic has brought global attention towards the readiness of biotechnology platforms for rapid development of immunogens to be used as vaccine antigens, in diagnostics and in fundamental research.. In the present study we provide a bioprocess that is both robust and industry-compatible for high level expression, efficient purification, and scale-up of IMT-CVAX, a prefusion-stabilized trimeric spike protein of SARS-CoV-2.. The recombinant IMT-CVAX protein was produced using stable cell pool of Chinese Hamster Ovary (CHO-S) cells that were banked in compliance with cGMP (Current Good Manufacturing Practices) regulations. Clone Assay:Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Control:Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Purification:Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Filtration:Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Affinity Chromatography:Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19 Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAV-P2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a Article Title: rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19. Article Snippet: The optimized sequence of the firefly luciferase gene Fluc was obtained from the vector pGL4.51[luc2/ CMV/Neo] (Promega). .. Both sequences were cloned into the rAAV–EGFP Control Vector plasmid instead of the EGFP gene at the EcoRI and XbaI restriction sites, thus obtaining the rAAVP2C5-Fc and rAAV-Luc plasmids, respectively. rAAV-P2C5-Fc and rAAV-Luc were produced by transient transfections of HEK293 cells (obtained from the cell cultures collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a |
![The SU BB1, used for the scale-up cultivations, is characterized by its flat bottom and features two 45°-pitched three-blade impellers spaced 70 mm apart. As clockwise and counter-clockwise stirring is possible (top view), the BB1 may be operated in either up- or down-pumping mode. Both impellers have a diameter ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${d}_{R}$$\end{document} d R ) of 50 mm, which, together with the maximum liquid height ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${H}_{L}$$\end{document} H L ) during operation of 160 mm, vessel diameter ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${D}_{R}$$\end{document} D R ) of 100 mm and impeller clearance to the base of 3.5 mm ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${h}_{R}$$\end{document} h R ), yield \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${d}_{R}/{D}_{R}$$\end{document} d R / D R , \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${H}_{L}/{D}_{R}$$\end{document} H L / D R , and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${h}_{R}/{D}_{R}$$\end{document} h R / D R ratios of 0.500, 1.600, and 0.035, respectively. Dissolved oxygen (DO) and pH control may be realized through a combination of headspace aeration and open-pipe sparging. At the same time, online monitoring of both values is facilitated through a built-in SU pH optode and a polarographic DO sensor port. During the experiments, the <t>bioreactor</t> was operated with either a BioFlo ® 320 bioprocess control system or with DASGIP Bioprocess Modules (Eppendorf AG, DE), with the growth surface provided by rigid, coated, non-porous, spherical MCs. Image partially created with Biorender.com](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_2619/pmc11802619/pmc11802619__253_2024_13372_Fig2_HTML.jpg)